什么原因导致性功能减退
Abstract
Using the atomic structures of the large ribosomal subunit fromHaloarcula marismortui and its complexes with two substrate analogs, we establish that the ribosome is a ribozyme and address the catalytic properties of its all-RNA active site. Both substrate analogs are contacted exclusively by conserved ribosomal RNA (rRNA) residues from domain V of 23S rRNA; there are no protein side-chain atoms closer than about 18 angstroms to the peptide bond being synthesized. The mechanism of peptide bond synthesis appears to resemble the reverse of the acylation step in serine proteases, with the base of A2486 (A2451 in Escherichia coli) playing the same general base role as histidine-57 in chymotrypsin. The unusual pK a (where K a is the acid dissociation constant) required for A2486 to perform this function may derive in part from its hydrogen bonding to G2482 (G2447 in E. coli), which also interacts with a buried phosphate that could stabilize unusual tautomers of these two bases. The polypeptide exit tunnel is largely formed by RNA but has significant contributions from proteins L4, L22, and L39e, and its exit is encircled by proteins L19, L22, L23, L24, L29, and L31e.
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百度 德上高速池祁段的建成将结束石台县不通高速的历史，也是我省四纵八横高速公路网规划中纵三(济南至祁门高速公路)的一段，对尽快完善全省路网结构，推动高速公路网络化、规模化，实现县县通高速具有重要意义。View all access options to continue reading this article.
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Science
Volume 289 | Issue 5481
11 August 2000
11 August 2000
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Received: 10 July 2000
Accepted: 24 July 2000
Published in print: 11 August 2000
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